KRISTY YOUNG JOHNSON, Ph.D.
Date of Birth: March 24, 1970
Home Address: 298 Stefan Dr.
Home Phone: 843-762-3821 Charleston, SC 29412
Office Address: Duckett Hall 316, Biology Dept. The Citadel 171 Moultrie St. Charleston, S.C. 29409
Phone: 843-953-7879
E-mail: johnsonk1@citadel.edu
EMPLOYMENT HISTORY
| August 2002 - present: | Assistant Professor at The Citadel - General Biology (lecture and laboratory) Microbiology (lecture and laboratory) Genetics (laboratory) Descriptive Histology (lecture and laboratory) |
| August 2001 - May 2002: | Visiting Assistant Professor at The Citadel - General Biology (lecture and laboratory) Microbiology (lecture and laboratory) |
| June 1998 - June 2001: | Post-doctoral fellow at the Medical University of South Carolina
- research topics: signal transduction pathways involving ras mode of Pseudomonas aeruginosa exoenzyme S transmission - supervisor: Timothy S. Vincent, Ph.D. |
| January 2001 - May 2001: | Adjunct faculty at the Citadel - Genetics (laboratory) - supervisor: Col. Robert E. Baldwin, Ph.D. |
| August 2000 -December 2000: | Adjunct faculty at the College of Charleston - General Chemistry (laboratory) - supervisor: James Deavor, Ph.D. |
| January 2000 - May 2000: | Adjunct faculty at the Citadel - Descriptive Histology (lecture and laboratory) - supervisor: Col. Robert E. Baldwin, Ph.D. |
EDUCATION
| 1992 - 1998: | Medical University of South Carolina, Charleston, S. C. Ph.D., department of Pathology and Laboratory Medicine, May, 1998 |
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| Research Topics: | Characterization of the phosphorylation of the Bcl-2 oncoprotein
Characterization of a protein antigen lost in apoptotic cell death Development of a novel nick translation protocol Screening of various human tissues for reactivity to the B3 antibody |
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| Awards: | 1998 Annual Meeting of the American Association for Cancer
Research - selected to participate in Poster Discussion Session 1997 MUSC Student Research Day, - 2nd place in "Ph.D. 3rd year +" category |
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| Dissertation: | Characterization and Analysis of the Hyperphosphorylation of the Bcl-2 Protein. | ||
| Advisor: | Mark C. Willingham, M.D. | ||
| 1988 - 1992: | Gardner-Webb University, Boiling Springs, N. C. B.S. in Chemistry with minors in Biology and Religious Education |
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| Honors and Awards: | Presidential Scholarship Dean's List |
Alpha Chi Beta Beta Beta |
Honors Program Graduated magna cum laude |
| Positions held: | Biology Department Assistant/Tutor (1989 - 1992) - prepared laboratory experiments for General Biology and Microbiology labs - supervised and assisted students during lab sessions - provided tutoring services for all students enrolled in General Biology |
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| Mathematics Department Tutor (1988 - 1989) - provided tutoring services for all students enrolled in College Algebra - supervised groups of 15 - 30 students daily |
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SEMINAR PRESENTATIONS
"Pseudomonas aeruginosa: A Common Yet Dangerous Species"
"Characterization and Analysis of the Hyperphosphorylation of the Bcl-2 Protein"
"Examining the Phosphorylation of the Bcl-2 Protein"
"Characterization and Analysis of the Functional Role of Phosphorylation of
the Bcl-2 Protein"
"Phosphorylation: An Overview" "Breast Cancer and BRCA1"
"Do Some Unique Histones Have a Role in Apoptosis?"
"Transfecting Genes Into Mammalian Cells"
PUBLICATIONS
Korey R. Johnson, Kristy Y. Johnson, Kevin P. Becker, Jacek Bielawski, Cungui Mao, and Lina M. Obeid. Role of Human Sphingosine-1-phosphate Phosphatase 1 in the Regulation of Intra- and Extracellular Sphingosine-1-phosphate Levels and Cell Viability. The Journal of Biological. Chemistry, Sep 2003; 278: 34541 - 34547.
Young, K.K. and Vincent, T.S. A Minimal FLAG Sequence Useful in the Functional Epitope Tagging of H-Ras. Biotechniques 32: 1270-1280.
Johnson, K.R., Young, K.K., and Fan, W.F. Antagonistic Interplay Between Antimitotic and G1/S Arresting Agents Observed in Experimental Combination Therapy. Clinical Cancer Research, 5: 2559-2565, 1999.
Collins, J.A., Schandl, C.A., Young, K.K., Vesely, J., and Willingham, M.C. Major DNA Fragmentation is a Late Event in Apoptosis. The Journal of Histochemistry and Cytochemistry, 45 (7): 923-934, 1997.
ABSTRACTS
1. Hollings Cancer Center 1st Annual Research Retreat. August 31, 2001. Mechanisms of Growth Suppression of Cancer Cells by the Monoterperne Perillyl Alcohol. Vincent, T.S., Johnson, K.Y., Re, G., Baker, V., Meier, K.E., and Meier, G.P.
2. Eighty-Ninth Annual Meeting of the American Association for Cancer Research. March 28 - April 1, 1998, New Orleans, LA. Bcl-2 Hyperphosphorylation Occurs in G2/M Phase of the Cell Cycle. Young, K.K., Johnson, K.R., Schandl, C.A., Li, S., Fan, W., and Willingham, M.C.
3. Thirty-Second Annual MUSC Student Research Day. November 7, 1997, Charleston,
SC.
a) Phosphorylation of Bcl-2 Coincides with G2/M Phase of the Cell Cycle. Young,
K.K., Johnson, K.R., Li, S., Schandl, C.A., Willingham, M.C., and Fan, W.
b) 5-Fluorouracil Antagonizes Paclitaxel Cytotoxicity Against Human Solid Tumor
Cells in vitro. Johnson, K.R., Miller, M.C., Young, K.K., and Fan, W.
4. Thiry-First Annual MUSC Student Research Day. November 1, 1996, Charleston, SC. The Isoelectric Point of Bcl-2. Young, K.K., Schandl, C.A., and Willingham, M.C.
SPECIALIZED TRAINING
Present - Citadel Academy of the Scholarship of Teaching, Learning, and Evaluation - Communication Across the Curriculum
Spring 2003 - attended "Critical Thinking" workshop by Dr. Laura Kaplan
Fall 2002 - attended "AD/HD: Nature and Management" workshop by Dr. Russell Barkley
Spring 2001 - completed Teaching Techniques course at Medical University of South Carolina
Spring 2000 - attended "Communication Across the Curriculum" workshop
RESEARCH SKILLS
| Isoelectric focusing | Mammalian cell culture |
| in vitro cell synchrony | Immunoblot analysis |
| Two-Dimensional gel electrophoresis | Nick translation assays |
| Site-directed mutagenesis | Immunohistochemistry |
| Protein immunoprecipitation | Transfection of genes into mammalian cells |
| PCR | Bacterial co-culture |
COMMUNITY SERVICE
Lowcountry Science Fair mentor
MUSC Children's Hospital volunteer
Ronald McDonald House volunteer
Communities in Schools volunteer
Red Cross blood donor